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Human E3 ubiquitin-protein ligase RNF5 (aa 1-180)

G16 Swiss Prot#

Q99942

G16 Protein names

E3 ubiquitin-protein ligase RNF5 (EC 6.3.2.-) (RING finger protein 5) (Ram1 homolog) (HsRma1) (Protein G16)

G16 Gene names

RNF5 G16 NG2 RMA1

Organism

Human

G16 Entry name

RNF5_HUMAN

G16 Codon optimized cDNA(gene) Clone

G16 Cat #

RPS0729

Tag

G16 contains A 6x histidine tag

G16 Formulation

Liquid

G16 Storage

Storage at -20℃ to −80℃ Upon receipt. Avoid repeated freeze-thaw cycles.

G16 Applications

Applications: 1. Codon-optimized cDNA(gene) sequence designed for protein production in both E.coli and Mammalian cell. The cDNA(gene) has been cloned into E.coli expression vector with 6x Histidine tag.2. May be used as RNAi assistance to restore the loss-of-function.

Recombinant G16 Protein

G16 Cat #

PS0729

G16 Source

recombinant

G16 Applications

Western Blot, SDS-Page or may be used for other applications determined by user

G16 Purity

>90%, as determined by SDS-PAGE under reducing conditions

G16 Activity

N/A

Tag

G16 Contains A 6x histidine tag

G16 Formulation

Liquid

G16 Storage

;. Storage at -20℃ to −80℃ Upon receipt. Avoid repeated freeze-thaw cycles.

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G16 family

-

G16 Subcellular locations

Membrane; Multi-pass membrane protein. Mitochondrion membrane. Endoplasmic reticulum membrane.

G16 FUNCTION According to UniProtKB Swiss Prot

FUNCTION: Has E2-dependent E3 ubiquitin-protein ligase activity. May function together with E2 ubiquitin-conjugating enzymes UBE2D1/UBCH5A and UBE2D2/UBC4. Mediates ubiquitination of PXN/paxillin and Salmonella type III secreted protein sopA. May be involved in regulation of cell motility and localization of PXN/paxillin. Mediates the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD; the ubiquitination appears to involve E2 ubiquitin-conjugating enzyme UBE2N. Mediates the 'Lys-48'-linked polyubiquitination of TMEM173 at 'Lys-150' leading to its proteasomal degradation; the ubiquitination occurrs in mitochondria after viral transfection and regulates antiviral responses.

G16 Sequence

DDBJ

EMBL

GenBank

G16 3D structure

ModBase

G16 interaction

STRING

G16 Proteomic

PRIDE

G16 Genome annotation

Ensembl

GeneID

KEGG

UCSC

G16 Organism specific

CTD

MGI

G16 Phylogenomics

eggNOG

GeneTree

HOGENOM

HOVERGEN

InParanoid

OrthoDB

PhylomeDB

OMA

G16 Gene expression

Bgee

ArrayExpress

Genevestigator

G16 Family and domain

InterPro

PROSITE

ProtoNet

FOR in vitro RESEARCH USE ONLY