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CLOBO Botulinum neurotoxin type A (aa 2-448)

atx Swiss Prot#

Q45894

atx Protein names

Botulinum neurotoxin type A (BoNT/A) (EC 3.4.24.69) (Bontoxilysin-A) (BOTOX) [Cleaved into: Botulinum neurotoxin A light chain; Botulinum neurotoxin A heavy chain]

atx Gene names

botA (atx) (bna)

Organism

Clostridium botulinum

atx Entry name

BXA2_CLOBO

atx Codon optimized cDNA(gene) Clone

atx Cat #

RPQ0056

Tag

atx contains A 6x histidine tag

atx Formulation

Liquid

atx Storage

Storage at -20℃ to −80℃ Upon receipt. Avoid repeated freeze-thaw cycles.

atx Applications

Applications: 1. Codon-optimized cDNA(gene) sequence designed for protein production in both E.coli and Mammalian cell. The cDNA(gene) has been cloned into E.coli expression vector with 6x Histidine tag.2. May be used as RNAi assistance to restore the loss-of-function.

Recombinant atx Protein

atx Cat #

PQ0056

atx Source

recombinant

atx Applications

Western Blot, SDS-Page or may be used for other applications determined by user

atx Purity

>90%, as determined by SDS-PAGE under reducing conditions

atx Activity

N/A

Tag

atx Contains A 6x histidine tag

atx Formulation

Liquid

atx Storage

;. Storage at -20℃ to −80℃ Upon receipt. Avoid repeated freeze-thaw cycles.

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atx family

Peptidase M27 family

atx Subcellular locations

Secreted. Host cytoplasm > host cytosol. Secreted. Host cell junction > host synapse > host presynaptic cell membrane; Multi-pass membrane protein.

atx FUNCTION According to UniProtKB Swiss Prot

FUNCTION: Inhibits acetylcholine release. The botulinum toxin binds with high affinity to peripheral neuronal presynaptic membrane to the secretory vesicle protein SV2. It binds directly to the largest luminal loop of the three isoforms SV2A, SV2B and SV2C. It is then internalized by receptor-mediated endocytosis. The C-terminus of the heavy chain (H) is responsible for the adherence of the toxin to the cell surface while the N-terminus mediates transport of the light chain from the endocytic vesicle to the cytosol. After translocation, the light chain (L) hydrolyzes the '197-Gln-|-Arg-198' bond in SNAP-25, thereby blocking neurotransmitter release. Inhibition of acetylcholine release results in flaccid paralysis, with frequent heart or respiratory failure (By similarity).

atx Sequence

DDBJ

EMBL

GenBank

atx 3D structure

ModBase

atx interaction

STRING

atx Proteomic

PRIDE

atx Genome annotation

Ensembl

GeneID

KEGG

UCSC

atx Organism specific

CTD

MGI

atx Phylogenomics

eggNOG

GeneTree

HOGENOM

HOVERGEN

InParanoid

OrthoDB

PhylomeDB

OMA

atx Gene expression

Bgee

ArrayExpress

Genevestigator

atx Family and domain

InterPro

PROSITE

ProtoNet

FOR in vitro RESEARCH USE ONLY