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Mouse Cell division protein kinase 4 (aa 1-303)

Crk3 Swiss Prot#

P30285

Crk3 Protein names

Cell division protein kinase 4 (EC 2.7.11.22) (Cyclin-dependent kinase 4) (PSK-J3) (CRK3)

Crk3 Gene names

Cdk4 (Crk3)

Organism

Mouse

Crk3 Entry name

CDK4_MOUSE

Crk3 Codon optimized cDNA(gene) Clone

Crk3 Cat #

RPU0154

Tag

Crk3 contains A 6x histidine tag

Crk3 Formulation

Liquid

Crk3 Storage

Storage at -20℃ to −80℃ Upon receipt. Avoid repeated freeze-thaw cycles.

Crk3 Applications

Applications: 1. Codon-optimized cDNA(gene) sequence designed for protein production in both E.coli and Mammalian cell. The cDNA(gene) has been cloned into E.coli expression vector with 6x Histidine tag.2. May be used as RNAi assistance to restore the loss-of-function.

Recombinant Crk3 Protein

Crk3 Cat #

PU0154

Crk3 Source

recombinant

Crk3 Applications

Western Blot, SDS-Page or may be used for other applications determined by user

Crk3 Purity

>90%, as determined by SDS-PAGE under reducing conditions

Crk3 Activity

N/A

Tag

Crk3 Contains A 6x histidine tag

Crk3 Formulation

Liquid

Crk3 Storage

;. Storage at -20℃ to −80℃ Upon receipt. Avoid repeated freeze-thaw cycles.

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Crk3 family

Protein kinase superfamily, CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily

Crk3 Subcellular locations

Cytoplasm. Nucleus. Membrane.

Crk3 FUNCTION According to UniProtKB Swiss Prot

FUNCTION: Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex (By similarity).

Crk3 Sequence

DDBJ

EMBL

GenBank

Crk3 3D structure

ModBase

Crk3 interaction

STRING

Crk3 Proteomic

PRIDE

Crk3 Genome annotation

Ensembl

GeneID

KEGG

UCSC

Crk3 Organism specific

CTD

MGI

Crk3 Phylogenomics

eggNOG

GeneTree

HOGENOM

HOVERGEN

InParanoid

OrthoDB

PhylomeDB

OMA

Crk3 Gene expression

Bgee

ArrayExpress

Genevestigator

Crk3 Family and domain

InterPro

PROSITE

ProtoNet

FOR in vitro RESEARCH USE ONLY