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Human 3 beta-hydroxysteroid dehydrogenase Delta 5 (aa 2-373)
3BH Swiss Prot#
P14060
3BH Protein names
3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1 (3-beta-HSD I) (Trophoblast antigen FDO161G) [Includes: 3-beta-hydroxy-Delta(5)-steroid dehydrogenase (EC 1.1.1.145) (3-beta-hydroxy-5-ene steroid dehydrogenase) (Progesterone reductase) Steroid Delta-isomerase (EC 5.3.3.1) (Delta-5-3-ketosteroid isomerase)]
3BH Gene names
HSD3B1 (3BH) (HSDB3A)
Organism
Homo sapiens (Human)
3BH Entry name
3BHS1_HUMAN
3BH Codon optimized cDNA(gene) Clone
3BH Cat #
RPM0074
Tag
3BH contains A 6x histidine tag
3BH Formulation
Liquid
3BH Storage
Storage at -20℃ to −80℃ Upon receipt. Avoid repeated freeze-thaw cycles.
3BH Applications
Applications: 1. Codon-optimized cDNA(gene) sequence designed for protein production in both E.coli and Mammalian cell. The cDNA(gene) has been cloned into E.coli expression vector with 6x Histidine tag.2. May be used as RNAi assistance to restore the loss-of-function.
Recombinant 3BH Protein
3BH Cat #
PM0074
3BH Source
recombinant
3BH Applications
Western Blot, SDS-Page or may be used for other applications determined by user
3BH Purity
>90%, as determined by SDS-PAGE under reducing conditions
3BH Activity
N/A
Tag
3BH Contains A 6x histidine tag
3BH Formulation
Liquid
3BH Storage
;. Storage at -20℃ to −80℃ Upon receipt. Avoid repeated freeze-thaw cycles.
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3BH family
3-beta-HSD family
3BH Subcellular locations
Endoplasmic reticulum membrane; Single-pass membrane protein. Mitochondrion membrane; Single-pass membrane protein.
3BH FUNCTION According to UniProtKB Swiss Prot
FUNCTION: 3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids. Efficiently catalyzes the transformation of pregnenolone to progesterone, 17-alpha-hydroxypregnenolone to 17-alpha-hydroxyprogesterone, DHEA to 4-androstenedione, dihydrotestosterone to 5-alpha-androstane-3 beta,17 beta-diol, dehydroepiandrosterone to androstenedione and 5-alpha-androstan-3 beta,17 beta-diol to 5-alpha-dihydrotestosterone.
3BH Sequence
DDBJ EMBL GenBank
3BH 3D structure
ModBase
3BH interaction
STRING
3BH Proteomic
PRIDE
3BH Genome annotation
Ensembl GeneID KEGG UCSC
3BH Organism specific
CTD MGI
3BH Phylogenomics
eggNOG GeneTree HOGENOM HOVERGEN

InParanoid OrthoDB PhylomeDB OMA
3BH Gene expression
Bgee ArrayExpress Genevestigator
3BH Family and domain
InterPro PROSITE ProtoNet
FOR in vitro RESEARCH USE ONLY