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| Human Adenylyltransferase and sulfurtransferase MOCS3 (aa 1-460) |
| UBA4 Swiss Prot# | | O95396 |
| UBA4 Protein names | | Adenylyltransferase and sulfurtransferase MOCS3 (Molybdenum cofactor synthesis protein 3) (Molybdopterin synthase sulfurylase) (MPT synthase sulfurylase) [Includes: Adenylyltransferase MOCS3 (EC 2.7.7.-) Sulfurtransferase MOCS3 (EC 2.8.1.-)] |
| UBA4 Gene names | | MOCS3 (UBA4) |
| Organism | | Homo sapiens (Human) |
| UBA4 Entry name | | MOCS3_HUMAN |
| UBA4 Codon optimized cDNA(gene) Clone |
| UBA4 Cat # | | RPM0009 |
| Tag | | UBA4 contains A 6x histidine tag |
| UBA4 Formulation | | Liquid |
| UBA4 Storage | | Storage at -20℃ to −80℃ Upon receipt. Avoid repeated freeze-thaw cycles. |
| UBA4 Applications | | Applications: 1. Codon-optimized cDNA(gene) sequence designed for protein production in both E.coli and Mammalian cell. The cDNA(gene) has been cloned into E.coli expression vector with 6x Histidine tag.2. May be used as RNAi assistance to restore the loss-of-function. |
| Recombinant UBA4 Protein |
| UBA4 Cat # | | PM0009 |
| UBA4 Source | | recombinant |
| UBA4 Applications | | Western Blot, SDS-Page or may be used for other applications determined by user |
| UBA4 Purity | | >90%, as determined by SDS-PAGE under reducing conditions |
| UBA4 Activity | | N/A |
| Tag | | UBA4 Contains A 6x histidine tag |
| UBA4 Formulation | | Liquid |
| UBA4 Storage | | ;. Storage at -20℃ to −80℃ Upon receipt. Avoid repeated freeze-thaw cycles. |
| Place Order for Recombinant UBA4 Products | | For pricing and availability Click Here >>> |
| UBA4 family | | HesA/moeB/thiF family, UBA4 subfamily |
| UBA4 Subcellular locations | | Cytoplasm > cytosol. |
| UBA4 FUNCTION According to UniProtKB Swiss Prot | | FUNCTION: Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers URM1 and MOCS2A. Its N-terminus first activates URM1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1 and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; NFS1 probably acting as a sulfur donor for thiocarboxylation reactions. |
| UBA4 Sequence | | DDBJ | EMBL | GenBank |
| UBA4 3D structure | | ModBase |
| UBA4 interaction | | STRING |
| UBA4 Proteomic | | PRIDE |
| UBA4 Genome annotation | | Ensembl | GeneID | KEGG | UCSC |
| UBA4 Organism specific | | CTD | MGI |
| UBA4 Phylogenomics | | eggNOG | GeneTree | HOGENOM | HOVERGEN |
| | InParanoid | OrthoDB | PhylomeDB | OMA |
| UBA4 Gene expression | | Bgee | ArrayExpress | Genevestigator |
| UBA4 Family and domain | | InterPro | PROSITE | ProtoNet |
| FOR in vitro RESEARCH USE ONLY |