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Human Adenylyltransferase and sulfurtransferase MOCS3 (aa 1-460)
UBA4 Swiss Prot#
O95396
UBA4 Protein names
Adenylyltransferase and sulfurtransferase MOCS3 (Molybdenum cofactor synthesis protein 3) (Molybdopterin synthase sulfurylase) (MPT synthase sulfurylase) [Includes: Adenylyltransferase MOCS3 (EC 2.7.7.-) Sulfurtransferase MOCS3 (EC 2.8.1.-)]
UBA4 Gene names
MOCS3 (UBA4)
Organism
Homo sapiens (Human)
UBA4 Entry name
MOCS3_HUMAN
UBA4 Codon optimized cDNA(gene) Clone
UBA4 Cat #
RPM0009
Tag
UBA4 contains A 6x histidine tag
UBA4 Formulation
Liquid
UBA4 Storage
Storage at -20℃ to −80℃ Upon receipt. Avoid repeated freeze-thaw cycles.
UBA4 Applications
Applications: 1. Codon-optimized cDNA(gene) sequence designed for protein production in both E.coli and Mammalian cell. The cDNA(gene) has been cloned into E.coli expression vector with 6x Histidine tag.2. May be used as RNAi assistance to restore the loss-of-function.
Recombinant UBA4 Protein
UBA4 Cat #
PM0009
UBA4 Source
recombinant
UBA4 Applications
Western Blot, SDS-Page or may be used for other applications determined by user
UBA4 Purity
>90%, as determined by SDS-PAGE under reducing conditions
UBA4 Activity
N/A
Tag
UBA4 Contains A 6x histidine tag
UBA4 Formulation
Liquid
UBA4 Storage
;. Storage at -20℃ to −80℃ Upon receipt. Avoid repeated freeze-thaw cycles.
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UBA4 family
HesA/moeB/thiF family, UBA4 subfamily
UBA4 Subcellular locations
Cytoplasm > cytosol.
UBA4 FUNCTION According to UniProtKB Swiss Prot
FUNCTION: Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers URM1 and MOCS2A. Its N-terminus first activates URM1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1 and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; NFS1 probably acting as a sulfur donor for thiocarboxylation reactions.
UBA4 Sequence
DDBJ EMBL GenBank
UBA4 3D structure
ModBase
UBA4 interaction
STRING
UBA4 Proteomic
PRIDE
UBA4 Genome annotation
Ensembl GeneID KEGG UCSC
UBA4 Organism specific
CTD MGI
UBA4 Phylogenomics
eggNOG GeneTree HOGENOM HOVERGEN

InParanoid OrthoDB PhylomeDB OMA
UBA4 Gene expression
Bgee ArrayExpress Genevestigator
UBA4 Family and domain
InterPro PROSITE ProtoNet
FOR in vitro RESEARCH USE ONLY