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Human Interferon-inducible double RNA-dependent (aa 1-313)

PACT Swiss Prot#

O75569

PACT Protein names

Interferon-inducible double stranded RNA-dependent protein kinase activator A (Protein kinase, interferon-inducible double stranded RNA-dependent activator) (Protein activator of the interferon-induced protein kinase) (PKR-associated protein X) (PKR-associating protein X)

PACT Gene names

PRKRA (PACT) (RAX) (HSD14) (HSD-14)

Organism

Human

PACT Entry name

PRKRA_HUMAN

PACT Codon optimized cDNA(gene) Clone

PACT Cat #

RPC0127

Tag

PACT contains A 6x histidine tag

PACT Formulation

Liquid

PACT Storage

Storage at -20℃ to −80℃ Upon receipt. Avoid repeated freeze-thaw cycles.

PACT Applications

Applications: 1. Codon-optimized cDNA(gene) sequence designed for protein production in both E.coli and Mammalian cell. The cDNA(gene) has been cloned into E.coli expression vector with 6x Histidine tag.2. May be used as RNAi assistance to restore the loss-of-function.

Recombinant PACT Protein

PACT Cat #

PC0127

PACT Source

recombinant

PACT Applications

Western Blot, SDS-Page or may be used for other applications determined by user

PACT Purity

>90%, as determined by SDS-PAGE under reducing conditions

PACT Activity

N/A

Tag

PACT Contains A 6x histidine tag

PACT Formulation

Liquid

PACT Storage

;. Storage at -20℃ to −80℃ Upon receipt. Avoid repeated freeze-thaw cycles.

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PACT family

PRKRA family

PACT Subcellular locations

Cytoplasm > perinuclear region.

PACT FUNCTION According to UniProtKB Swiss Prot

FUNCTION: Activates EIF2AK2/PKR in the absence of double stranded RNA (dsRNA), leading to phosphorylation of EIF2S1/EFI2-alpha and inhibition of translation and induction of apoptosis. Required for siRNA production by DICER1 and for subsequent siRNA-mediated post-transcriptional gene silencing. Does not seem to be required for processing of pre-miRNA to miRNA by DICER1.

PACT Sequence

DDBJ

EMBL

GenBank

PACT 3D structure

ModBase

PACT interaction

STRING

PACT Proteomic

PRIDE

PACT Genome annotation

Ensembl

GeneID

KEGG

UCSC

PACT Organism specific

CTD

MGI

PACT Phylogenomics

eggNOG

GeneTree

HOGENOM

HOVERGEN

InParanoid

OrthoDB

PhylomeDB

OMA

PACT Gene expression

Bgee

ArrayExpress

Genevestigator

PACT Family and domain

InterPro

PROSITE

ProtoNet

FOR in vitro RESEARCH USE ONLY