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Human Serine threonine-protein kinase Chk1 (aa 1-476)

CHK1 Swiss Prot#

O14757

CHK1 Protein names

Serine/threonine-protein kinase Chk1 (EC 2.7.11.1)

CHK1 Gene names

CHEK1 (CHK1)

Organism

Human

CHK1 Entry name

CHK1_HUMAN

CHK1 Codon optimized cDNA(gene) Clone

CHK1 Cat #

RPT0307

Tag

CHK1 contains A 6x histidine tag

CHK1 Formulation

Liquid

CHK1 Storage

Storage at -20℃ to −80℃ Upon receipt. Avoid repeated freeze-thaw cycles.

CHK1 Applications

Applications: 1. Codon-optimized cDNA(gene) sequence designed for protein production in both E.coli and Mammalian cell. The cDNA(gene) has been cloned into E.coli expression vector with 6x Histidine tag.2. May be used as RNAi assistance to restore the loss-of-function.

Recombinant CHK1 Protein

CHK1 Cat #

PT0307

CHK1 Source

recombinant

CHK1 Applications

Western Blot, SDS-Page or may be used for other applications determined by user

CHK1 Purity

>90%, as determined by SDS-PAGE under reducing conditions

CHK1 Activity

N/A

Tag

CHK1 Contains A 6x histidine tag

CHK1 Formulation

Liquid

CHK1 Storage

;. Storage at -20℃ to −80℃ Upon receipt. Avoid repeated freeze-thaw cycles.

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CHK1 family

Protein kinase superfamily, CAMK Ser/Thr protein kinase family, NIM1 subfamily

CHK1 Subcellular locations

Nucleus. Cytoplasm. Cytoplasm > cytoskeleton > centrosome.

CHK1 FUNCTION According to UniProtKB Swiss Prot

FUNCTION: Required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. Recognizes the substrate consensus sequence [R-X-X-S/T]. Binds to and phosphorylates CDC25A, CDC25B and CDC25C. Phosphorylation of CDC25A at 'Ser-178' and 'Thr-507' and phosphorylation of CDC25C at 'Ser-216' creates binding sites for 14-3-3 proteins which inhibit CDC25A and CDC25C. Phosphorylation of CDC25A at 'Ser-76', 'Ser-124', 'Ser-178', 'Ser-279' and 'Ser-293' promotes proteolysis of CDC25A. Inhibition of CDC25 activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. Binds to and phosphorylates RAD51 at 'Thr-309', which may enhance the association of RAD51 with chromatin and promote DNA repair by homologous recombination. Binds to and phosphorylates TLK1 at 'Ser-743', which prevents the TLK1-dependent phosphorylation of the chromatin assembly factor ASF1A. This may affect chromatin assembly during S phase or DNA repair. May also phosphorylate multiple sites within the C-terminus of TP53, which promotes activation of TP53 by acetylation and enhances suppression of cellular proliferation.

CHK1 Sequence

DDBJ

EMBL

GenBank

CHK1 3D structure

ModBase

CHK1 interaction

STRING

CHK1 Proteomic

PRIDE

CHK1 Proteomic

Ensembl

GeneID

KEGG

UCSC

CHK1 Organism specific

CTD

MGI

CHK1 Phylogenomics

eggNOG

GeneTree

HOGENOM

HOVERGEN

InParanoid

OrthoDB

PhylomeDB

OMA

CHK1 Gene expression

Bgee

ArrayExpress

Genevestigator

CHK1 Family and domain

InterPro

PROSITE

ProtoNet

FOR in vitro RESEARCH USE ONLY