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Human Serine threonine-protein kinase Nek2 (aa 1-445)

NLK1 Swiss Prot#

P51955

NLK1 Protein names

Serine/threonine-protein kinase Nek2 (EC 2.7.11.1) (NimA-related protein kinase 2) (NimA-like protein kinase 1) (HSPK 21)

NLK1 Gene names

NEK2 (NLK1)

Organism

Human

NLK1 Entry name

NEK2_HUMAN

NLK1 Codon optimized cDNA(gene) Clone

NLK1 Cat #

RPT0280

Tag

NLK1 contains A 6x histidine tag

NLK1 Formulation

Liquid

NLK1 Storage

Storage at -20℃ to −80℃ Upon receipt. Avoid repeated freeze-thaw cycles.

NLK1 Applications

Applications: 1. Codon-optimized cDNA(gene) sequence designed for protein production in both E.coli and Mammalian cell. The cDNA(gene) has been cloned into E.coli expression vector with 6x Histidine tag.2. May be used as RNAi assistance to restore the loss-of-function.

Recombinant NLK1 Protein

NLK1 Cat #

PT0280

NLK1 Source

recombinant

NLK1 Applications

Western Blot, SDS-Page or may be used for other applications determined by user

NLK1 Purity

>90%, as determined by SDS-PAGE under reducing conditions

NLK1 Activity

N/A

Tag

NLK1 Contains A 6x histidine tag

NLK1 Formulation

Liquid

NLK1 Storage

;. Storage at -20℃ to −80℃ Upon receipt. Avoid repeated freeze-thaw cycles.

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NLK1 family

Protein kinase superfamily, NEK Ser/Thr protein kinase family, NIMA subfamily

NLK1 Subcellular locations

Nucleus. Chromosome > centromere. Chromosome > centromere > kinetochore. Nucleus > nucleolus. Cytoplasm.

NLK1 FUNCTION According to UniProtKB Swiss Prot

FUNCTION: Protein kinase that is involved in mitotic regulation. Integral component of the mitotic spindle-assembly checkpoint which is necessary for proper chromosome segregation during metaphase-anaphase transition. Required for association of MAD2L1 to kinetochore. Phosphorylates SGOL1. May have a role at the G2-M transition. May also play a role in meiosis. Isoform 1 but not isoform 2 appears to play a role in centrosome splitting. Isoform 1 phosphorylates and activates NEK11 in G1/S-arrested cells. Isoform 2, which is not present in the nucleolus, does not.

NLK1 Sequence

DDBJ

EMBL

GenBank

NLK1 3D structure

ModBase

NLK1 interaction

STRING

NLK1 Proteomic

PRIDE

NLK1 Proteomic

Ensembl

GeneID

KEGG

UCSC

NLK1 Organism specific

CTD

MGI

NLK1 Phylogenomics

eggNOG

GeneTree

HOGENOM

HOVERGEN

InParanoid

OrthoDB

PhylomeDB

OMA

NLK1 Gene expression

Bgee

ArrayExpress

Genevestigator

NLK1 Family and domain

InterPro

PROSITE

ProtoNet

FOR in vitro RESEARCH USE ONLY