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Mouse Guanine nucleotide-binding protein G(t) alpha-3 (aa 1-354)

Gnat3 Swiss Prot#

Q3V3I2

Gnat3 Protein names

Guanine nucleotide-binding protein G(t) subunit alpha-3 (Gustducin alpha-3 chain)

Gnat3 Gene names

Gnat3

Organism

Mouse

Gnat3 Entry name

GNAT3_MOUSE

Gnat3 Codon optimized cDNA(gene) Clone

Gnat3 Cat #

RPX1378

Tag

Gnat3 contains A 6x histidine tag

Gnat3 Formulation

Liquid

Gnat3 Storage

Storage at -20℃ to −80℃ Upon receipt. Avoid repeated freeze-thaw cycles.

Gnat3 Applications

Applications: 1. Codon-optimized cDNA(gene) sequence designed for protein production in both E.coli and Mammalian cell. The cDNA(gene) has been cloned into E.coli expression vector with 6x Histidine tag.2. May be used as RNAi assistance to restore the loss-of-function.

Recombinant Gnat3 Protein

Gnat3 Cat #

PX1378

Gnat3 Source

recombinant

Gnat3 Applications

Western Blot, SDS-Page or may be used for other applications determined by user

Gnat3 Purity

>90%, as determined by SDS-PAGE under reducing conditions

Gnat3 Activity

N/A

Tag

Gnat3 Contains A 6x histidine tag

Gnat3 Formulation

Liquid

Gnat3 Storage

;. Storage at -20℃ to −80℃ Upon receipt. Avoid repeated freeze-thaw cycles.

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Gnat3 family

G-alpha family, G(i/o/t/z) subfamily

Gnat3 Subcellular locations

Cytoplasm.

Gnat3 FUNCTION According to UniProtKB Swiss Prot

FUNCTION: Guanine nucleotide-binding protein (G protein) alpha subunit playing a prominent role in bitter and sweet taste transduction as well as in umami (monosodium glutamate, monopotassium glutamate, and inosine monophosphate) taste transduction. Transduction by this alpha subunit involves coupling of specific cell-surface receptors with a cGMP-phosphodiesterase; Activation of phosphodiesterase lowers intracellular levels of cAMP and cGMP which may open a cyclic nucleotide-suppressible cation channel leading to influx of calcium, ultimately leading to release of neurotransmitter. Indeed, denatonium and strychnine induce transient reduction in cAMP and cGMP in taste tissue, whereas this decrease is inhibited by GNAT3 antibody. Gustducin heterotrimer transduces response to bitter and sweet compounds via regulation of phosphodiesterase for alpha subunit, as well as via activation of phospholipase C for beta and gamma subunits, with ultimate increase inositol trisphosphate and increase of intracellular Calcium. GNAT3 can functionally couple to taste receptors to transmit intracellular signal: receptor heterodimer TAS1R2/TAS1R3 senses sweetness and TAS1R1/TAS1R3 transduces umami taste, whereas the T2R family GPCRs act as bitter sensors. Functions also as lumenal sugar sensors in the gut to control the expression of the Na+-glucose transporter SGLT1 in response to dietaty sugar, as well as the secretion of Glucagon-like peptide-1, GLP-1 and glucose-dependent insulinotropic polypeptide, GIP. Thus, may modulate the gut capacity to absorb sugars, with implications in malabsorption syndromes and diet-related disorders including diabetes and obesity.

Gnat3 Sequence

DDBJ

EMBL

GenBank

Gnat3 3D structure

ModBase

Gnat3 interaction

STRING

Gnat3 Proteomic

PRIDE

Gnat3 Genome annotation

Ensembl

GeneID

KEGG

UCSC

Gnat3 Organism specific

CTD

MGI

Gnat3 Phylogenomics

eggNOG

GeneTree

HOGENOM

HOVERGEN

InParanoid

OrthoDB

PhylomeDB

OMA

Gnat3 Gene expression

Bgee

ArrayExpress

Genevestigator

Gnat3 Family and domain

InterPro

PROSITE

ProtoNet

FOR in vitro RESEARCH USE ONLY