All rights
reserved
|
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
|
||||||||||||||||||||||||||||||||||
|
|||||||||||||
|
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Human Non-POU domain-containing octamer-binding (aa 1-471) |
| NRB54 Swiss Prot# | | Q15233 |
| NRB54 Protein names | | Non-POU domain-containing octamer-binding protein (NonO protein) (54 kDa nuclear RNA- and DNA-binding protein) (p54(nrb)) (p54nrb) (55 kDa nuclear protein) (NMT55) (DNA-binding p52/p100 complex, 52 kDa subunit) |
| NRB54 Gene names | | NONO (NRB54) |
| Organism | | Human |
| NRB54 Entry name | | NONO_HUMAN |
| NRB54 Codon optimized cDNA(gene) Clone |
| NRB54 Cat # | | RPR0562 |
| Tag | | NRB54 contains A 6x histidine tag |
| NRB54 Formulation | | Liquid |
| NRB54 Storage | | Storage at -20℃ to −80℃ Upon receipt. Avoid repeated freeze-thaw cycles. |
| NRB54 Applications | | Applications: 1. Codon-optimized cDNA(gene) sequence designed for protein production in both E.coli and Mammalian cell. The cDNA(gene) has been cloned into E.coli expression vector with 6x Histidine tag.2. May be used as RNAi assistance to restore the loss-of-function. |
| Recombinant NRB54 Protein |
| NRB54 Cat # | | PR0562 |
| NRB54 Source | | recombinant |
| NRB54 Applications | | Western Blot, SDS-Page or may be used for other applications determined by user |
| NRB54 Purity | | >90%, as determined by SDS-PAGE under reducing conditions |
| NRB54 Activity | | N/A |
| Tag | | NRB54 Contains A 6x histidine tag |
| NRB54 Formulation | | Liquid |
| NRB54 Storage | | ;. Storage at -20℃ to −80℃ Upon receipt. Avoid repeated freeze-thaw cycles. |
| Place Order for Recombinant NRB54 Products | | For pricing and availability Click Here >>> |
| NRB54 family | | - |
| NRB54 Subcellular locations | | Nucleus. |
| NRB54 FUNCTION According to UniProtKB Swiss Prot | | FUNCTION: DNA- and RNA binding protein, involved in several nuclear processes. Binds the conventional octamer sequence in double stranded DNA. Also binds single-stranded DNA and RNA at a site independent of the duplex site (By similarity). Involved in pre-mRNA splicing, probably as an heterodimer with SFPQ. Interacts with U5 snRNA, probably by binding to a purine-rich sequence located on the 3' side of U5 snRNA stem 1b. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs. The SFPQ-NONO heteromer may be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1. The SFPQ-NONO heteromer may be involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination and may stabilize paired DNA ends. In vitro, the complex strongly stimulates DNA end joining, binds directly to the DNA substrates and cooperates with the Ku70/G22P1-Ku80/XRCC5 (Ku) dimer to establish a functional preligation complex. Nono is involved in transcriptional regulation. The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional avtivity. NONO binds to an enhancer element in long terminal repeats of endogenous intracisternal A particles (IAPs) and activates transcription (By similarity). |
| NRB54 Sequence | | DDBJ | EMBL | GenBank |
| NRB54 3D structure | | ModBase |
| NRB54 interaction | | STRING |
| NRB54 Proteomic | | PRIDE |
| NRB54 Genome annotation | | Ensembl | GeneID | KEGG | UCSC |
| NRB54 Organism specific | | CTD | MGI |
| NRB54 Phylogenomics | | eggNOG | GeneTree | HOGENOM | HOVERGEN |
| | InParanoid | OrthoDB | PhylomeDB | OMA |
| NRB54 Gene expression | | Bgee | ArrayExpress | Genevestigator |
| NRB54 Family and domain | | InterPro | PROSITE | ProtoNet |
| FOR in vitro RESEARCH USE ONLY |
