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Mouse Carbohydrate sulfotransferase 4 (aa 1-388)

Gst3 Swiss Prot#

Q9R1I1

Gst3 Protein names

Carbohydrate sulfotransferase 4 (EC 2.8.2.-) (N-acetylglucosamine 6-O-sulfotransferase 2) (GlcNAc6ST-2) (High endothelial cells N-acetylglucosamine 6-O-sulfotransferase) (HEC-GlcNAc6ST) (L-selectin ligand sulfotransferase) (LSST) (Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 3) (GST-3)

Gst3 Gene names

Chst4 (Gst3)

Organism

Mouse

Gst3 Entry name

CHST4_MOUSE

Gst3 Codon optimized cDNA(gene) Clone

Gst3 Cat #

RPR0186

Tag

Gst3 contains A 6x histidine tag

Gst3 Formulation

Liquid

Gst3 Storage

Storage at -20℃ to −80℃ Upon receipt. Avoid repeated freeze-thaw cycles.

Gst3 Applications

Applications: 1. Codon-optimized cDNA(gene) sequence designed for protein production in both E.coli and Mammalian cell. The cDNA(gene) has been cloned into E.coli expression vector with 6x Histidine tag.2. May be used as RNAi assistance to restore the loss-of-function.

Recombinant Gst3 Protein

Gst3 Cat #

PR0186

Gst3 Source

recombinant

Gst3 Applications

Western Blot, SDS-Page or may be used for other applications determined by user

Gst3 Purity

>90%, as determined by SDS-PAGE under reducing conditions

Gst3 Activity

N/A

Tag

Gst3 Contains A 6x histidine tag

Gst3 Formulation

Liquid

Gst3 Storage

;. Storage at -20℃ to −80℃ Upon receipt. Avoid repeated freeze-thaw cycles.

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Gst3 family

Sulfotransferase 1 family, Gal/GlcNAc/GalNAc subfamily

Gst3 Subcellular locations

Golgi apparatus membrane; Single-pass type II membrane protein.

Gst3 FUNCTION According to UniProtKB Swiss Prot

FUNCTION: Catalyzes the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues within mucin-associated glycans that ultimately serve as L-selectin ligands. L-selectin ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation. Participates in biosynthesis of L-selectin ligand sialyl 6-sulfo Lewis X on L-selectin counter receptors CD43, GlyCAM-1 and MAdCAM-1. Also involved in biosynthesis of L-selectin ligand recognized by MECA-79 antibody. Plays a central role in lymphocyte trafficking during chronic inflammation. Has a catalytic preference for core 2-branched mucin-type O-glycans. Can use GlcNAcbeta1-6[Galbeta1-3]GalNAc-pNP (core 2), GlcNAcbeta1-6ManOMe and GlcNAcbeta1-2Man oligosaccharide structures as acceptors. Has also activity toward core 3 of GlcNAcbeta1-3GalNAc-pNP. Its substrate specificity may be influenced by its subcellular location.

Gst3 Sequence

DDBJ

EMBL

GenBank

Gst3 3D structure

ModBase

Gst3 interaction

STRING

Gst3 Proteomic

PRIDE

Gst3 Genome annotation

Ensembl

GeneID

KEGG

UCSC

Gst3 Organism specific

CTD

MGI

Gst3 Phylogenomics

eggNOG

GeneTree

HOGENOM

HOVERGEN

InParanoid

OrthoDB

PhylomeDB

OMA

Gst3 Gene expression

Bgee

ArrayExpress

Genevestigator

Gst3 Family and domain

InterPro

PROSITE

ProtoNet

FOR in vitro RESEARCH USE ONLY