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Human Replication A 70 kDa DNA-binding subunit (aa 1-616 )

REPA1 Swiss Prot#

P27694

REPA1 Protein names

Replication protein A 70 kDa DNA-binding subunit (RP-A p70) (Replication factor A protein 1) (RF-A protein 1) (Single-stranded DNA-binding protein)

REPA1 Gene names

RPA1 REPA1 RPA70

Organism

Homo sapiens (Human)

REPA1 Entry name

RFA1_HUMAN

REPA1 Codon optimized cDNA(gene) Clone

REPA1 Cat #

RPY1525

Tag

REPA1 contains A 6x histidine tag

REPA1 Formulation

Liquid

REPA1 Storage

Storage at -20℃ to −80℃ Upon receipt. Avoid repeated freeze-thaw cycles.

REPA1 Applications

Applications: 1. Codon-optimized cDNA(gene) sequence designed for protein production in both E.coli and Mammalian cell. The cDNA(gene) has been cloned into E.coli expression vector with 6x Histidine tag.2. May be used as RNAi assistance to restore the loss-of-function.

Recombinant REPA1 Protein

REPA1 Cat #

PY1525

REPA1 Source

recombinant

REPA1 Applications

Western Blot, SDS-Page or may be used for other applications determined by user

REPA1 Purity

>90%, as determined by SDS-PAGE under reducing conditions

REPA1 Activity

N/A

Tag

REPA1 Contains A 6x histidine tag

REPA1 Formulation

Liquid

REPA1 Storage

;. Storage at -20℃ to −80℃ Upon receipt. Avoid repeated freeze-thaw cycles.

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REPA1 family

Replication factor A protein 1 family

REPA1 Subcellular locations

Nucleus.

REPA1 FUNCTION According to UniProtKB Swiss Prot

FUNCTION: Plays an essential role in several cellular processes in DNA metabolism including replication, recombination and DNA repair. Binds and subsequently stabilizes single-stranded DNA intermediates and thus prevents complementary DNA from reannealing.; FUNCTION: Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.

REPA1 Sequence

DDBJ

EMBL

GenBank

REPA1 3D structure

ModBase

REPA1 interaction

STRING

REPA1 Proteomic

PRIDE

REPA1 Genome annotation

Ensembl

GeneID

KEGG

UCSC

REPA1 Organism specific

CTD

MGI

REPA1 Phylogenomics

eggNOG

GeneTree

HOGENOM

HOVERGEN

InParanoid

OrthoDB

PhylomeDB

OMA

REPA1 Gene expression

Bgee

ArrayExpress

Genevestigator

REPA1 Family and domain

InterPro

PROSITE

ProtoNet

FOR in vitro RESEARCH USE ONLY