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Rat Rho guanine nucleotide exchange factor 25 (aa 1-579 )

Geft Swiss Prot#

Q6P720

Geft Protein names

Rho guanine nucleotide exchange factor 25 (Guanine nucleotide exchange factor GEFT) (Rac/Cdc42/Rho exchange factor GEFT) (RhoA/Rac/Cdc42 guanine nucleotide exchange factor GEFT)

Geft Gene names

Arhgef25 Geft

Organism

Rattus norvegicus (Rat)

Geft Entry name

ARHGP_RAT

Geft Codon optimized cDNA(gene) Clone

Geft Cat #

RPY1414

Tag

Geft contains A 6x histidine tag

Geft Formulation

Liquid

Geft Storage

Storage at -20℃ to −80℃ Upon receipt. Avoid repeated freeze-thaw cycles.

Geft Applications

Applications: 1. Codon-optimized cDNA(gene) sequence designed for protein production in both E.coli and Mammalian cell. The cDNA(gene) has been cloned into E.coli expression vector with 6x Histidine tag.2. May be used as RNAi assistance to restore the loss-of-function.

Recombinant Geft Protein

Geft Cat #

PY1414

Geft Source

recombinant

Geft Applications

Western Blot, SDS-Page or may be used for other applications determined by user

Geft Purity

>90%, as determined by SDS-PAGE under reducing conditions

Geft Activity

N/A

Tag

Geft Contains A 6x histidine tag

Geft Formulation

Liquid

Geft Storage

;. Storage at -20℃ to −80℃ Upon receipt. Avoid repeated freeze-thaw cycles.

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Geft family

-

Geft Subcellular locations

Cytoplasm > myofibril > sarcomere. Cell membrane.

Geft FUNCTION According to UniProtKB Swiss Prot

FUNCTION: May play a role in actin cytoskeleton reorganization in different tissues since its activation induces formation of actin stress fibers. It works as a guanine nucleotide exchange factor for Rho family of small GTPases. Links specifically G alpha q/11-coupled receptors to RHOA activation. May be an important regulator of processes involved in axon and dendrite formation. In neurons seems to be an exchange factor primarily for RAC1. Involved in skeletal myogenesis (By similarity).

Geft Sequence

DDBJ

EMBL

GenBank

Geft 3D structure

ModBase

Geft interaction

STRING

Geft Proteomic

PRIDE

Geft Genome annotation

Ensembl

GeneID

KEGG

UCSC

Geft Organism specific

CTD

MGI

Geft Phylogenomics

eggNOG

GeneTree

HOGENOM

HOVERGEN

InParanoid

OrthoDB

PhylomeDB

OMA

Geft Gene expression

Bgee

ArrayExpress

Genevestigator

Geft Family and domain

InterPro

PROSITE

ProtoNet

FOR in vitro RESEARCH USE ONLY