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Human Na(+) H(+) exchange regulatory c actor NHE-RF1 (aa 1-358 )

NHERF Swiss Prot#

O14745

NHERF Protein names

Na(+)/H(+) exchange regulatory cofactor NHE-RF1 (NHERF-1) (Ezrin-radixin-moesin-binding phosphoprotein 50) (EBP50) (Regulatory cofactor of Na(+)/H(+) exchanger) (Sodium-hydrogen exchanger regulatory factor 1) (Solute carrier family 9 isoform A3 regulatory factor 1)

NHERF Gene names

SLC9A3R1 NHERF NHERF1

Organism

Homo sapiens (Human)

NHERF Entry name

NHRF1_HUMAN

NHERF Codon optimized cDNA(gene) Clone

NHERF Cat #

RPY0716

Tag

NHERF contains A 6x histidine tag

NHERF Formulation

Liquid

NHERF Storage

Storage at -20℃ to −80℃ Upon receipt. Avoid repeated freeze-thaw cycles.

NHERF Applications

Applications: 1. Codon-optimized cDNA(gene) sequence designed for protein production in both E.coli and Mammalian cell. The cDNA(gene) has been cloned into E.coli expression vector with 6x Histidine tag.2. May be used as RNAi assistance to restore the loss-of-function.

Recombinant NHERF Protein

NHERF Cat #

PY0716

NHERF Source

recombinant

NHERF Applications

Western Blot, SDS-Page or may be used for other applications determined by user

NHERF Purity

>90%, as determined by SDS-PAGE under reducing conditions

NHERF Activity

N/A

Tag

NHERF Contains A 6x histidine tag

NHERF Formulation

Liquid

NHERF Storage

;. Storage at -20℃ to −80℃ Upon receipt. Avoid repeated freeze-thaw cycles.

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NHERF family

-

NHERF Subcellular locations

Cytoplasm. Apical cell membrane. Endomembrane system; Peripheral membrane protein. Cell projection > filopodium. Cell projection > ruffle. Cell projection > microvillus.

NHERF FUNCTION According to UniProtKB Swiss Prot

FUNCTION: Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for recycling of internalized ADRB2. Was first known to play a role in the regulation of the activity and subcellular location of SLC9A3. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May enhance Wnt signaling. May participate in HTR4 targeting to microvilli (By similarity). Interacts with MCC.

NHERF Sequence

DDBJ

EMBL

GenBank

NHERF 3D structure

ModBase

NHERF interaction

STRING

NHERF Proteomic

PRIDE

NHERF Genome annotation

Ensembl

GeneID

KEGG

UCSC

NHERF Organism specific

CTD

MGI

NHERF Phylogenomics

eggNOG

GeneTree

HOGENOM

HOVERGEN

InParanoid

OrthoDB

PhylomeDB

OMA

NHERF Gene expression

Bgee

ArrayExpress

Genevestigator

NHERF Family and domain

InterPro

PROSITE

ProtoNet

FOR in vitro RESEARCH USE ONLY