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Mouse Target rapamycin complex subunit LST8 (aa 1-326 )

Gbl Swiss Prot#

Q9DCJ1

Gbl Protein names

Target of rapamycin complex subunit LST8 (TORC subunit LST8) (G protein beta subunit-like) (Protein GbetaL) (Mammalian lethal with SEC13 protein 8) (mLST8)

Gbl Gene names

Mlst8 Gbl Lst8

Organism

Mus musculus (Mouse)

Gbl Entry name

LST8_MOUSE

Gbl Codon optimized cDNA(gene) Clone

Gbl Cat #

RPY0603

Tag

Gbl contains A 6x histidine tag

Gbl Formulation

Liquid

Gbl Storage

Storage at -20℃ to −80℃ Upon receipt. Avoid repeated freeze-thaw cycles.

Gbl Applications

Applications: 1. Codon-optimized cDNA(gene) sequence designed for protein production in both E.coli and Mammalian cell. The cDNA(gene) has been cloned into E.coli expression vector with 6x Histidine tag.2. May be used as RNAi assistance to restore the loss-of-function.

Recombinant Gbl Protein

Gbl Cat #

PY0603

Gbl Source

recombinant

Gbl Applications

Western Blot, SDS-Page or may be used for other applications determined by user

Gbl Purity

>90%, as determined by SDS-PAGE under reducing conditions

Gbl Activity

N/A

Tag

Gbl Contains A 6x histidine tag

Gbl Formulation

Liquid

Gbl Storage

;. Storage at -20℃ to −80℃ Upon receipt. Avoid repeated freeze-thaw cycles.

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Gbl family

WD repeat LST8 family

Gbl Subcellular locations

Cytoplasm.

Gbl FUNCTION According to UniProtKB Swiss Prot

FUNCTION: Subunit of both mTORC1 and mTORC2, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino-acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, LST8 interacts directly with MTOR and enhances its kinase activity. In nutrient-poor conditions, stabilizes the MTOR-RPTOR interaction and favors RPTOR-mediated inhibition of MTOR activity. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'.

Gbl Sequence

DDBJ

EMBL

GenBank

Gbl 3D structure

ModBase

Gbl interaction

STRING

Gbl Proteomic

PRIDE

Gbl Genome annotation

Ensembl

GeneID

KEGG

UCSC

Gbl Organism specific

CTD

MGI

Gbl Phylogenomics

eggNOG

GeneTree

HOGENOM

HOVERGEN

InParanoid

OrthoDB

PhylomeDB

OMA

Gbl Gene expression

Bgee

ArrayExpress

Genevestigator

Gbl Family and domain

InterPro

PROSITE

ProtoNet

FOR in vitro RESEARCH USE ONLY