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Rat SPARC (aa 18-301)

Sparc Swiss Prot#

P16975

Sparc Protein names

SPARC (Secreted protein acidic and rich in cysteine) (Osteonectin) (ON) (Basement-membrane protein 40) (BM-40)

Sparc Gene names

Sparc

Organism

Rat

Sparc Entry name

SPRC_RAT

Sparc Codon optimized cDNA(gene) Clone

Sparc Cat #

RPA0921

Tag

Sparc contains A 6x histidine tag

Sparc Formulation

Liquid

Sparc Storage

Storage at -20℃ to −80℃ Upon receipt. Avoid repeated freeze-thaw cycles.

Sparc Applications

Applications: 1. Codon-optimized cDNA(gene) sequence designed for protein production in both E.coli and Mammalian cell. The cDNA(gene) has been cloned into E.coli expression vector with 6x Histidine tag.2. May be used as RNAi assistance to restore the loss-of-function.

Recombinant Sparc Protein

Sparc Cat #

PA0921

Sparc Source

recombinant

Sparc Applications

Western Blot, SDS-Page or may be used for other applications determined by user

Sparc Purity

>90%, as determined by SDS-PAGE under reducing conditions

Sparc Activity

N/A

Tag

Sparc Contains A 6x histidine tag

Sparc Formulation

Liquid

Sparc Storage

;. Storage at -20℃ to −80℃ Upon receipt. Avoid repeated freeze-thaw cycles.

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Sparc family

SPARC family

Sparc Subcellular locations

Secreted > extracellular space > extracellular matrix > basement membrane.

Sparc FUNCTION According to UniProtKB Swiss Prot

FUNCTION: Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.

Sparc Sequence

DDBJ

EMBL

GenBank

Sparc 3D structure

ModBase

Sparc interaction

STRING

Sparc Proteomic

PRIDE

Sparc Genome annotation

Ensembl

GeneID

KEGG

UCSC

Sparc Organism specific

CTD

MGI

Sparc Phylogenomics

eggNOG

GeneTree

HOGENOM

HOVERGEN

InParanoid

OrthoDB

PhylomeDB

OMA

Sparc Gene expression

Bgee

ArrayExpress

Genevestigator

Sparc Family and domain

InterPro

PROSITE

ProtoNet

FOR in vitro RESEARCH USE ONLY