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Mouse E3 ubiquitin-protein ligase RNF128 (aa 29-428)

Grail Swiss Prot#

Q9D304

Grail Protein names

E3 ubiquitin-protein ligase RNF128 (EC 6.3.2.-) (RING finger protein 128) (Gene related to anergy in lymphocytes protein) (Goliath-related E3 ubiquitin-protein ligase 1)

Grail Gene names

Rnf128 (Grail) (Greul1) (MNCb-3816)

Organism

Mouse

Grail Entry name

RN128_MOUSE

Grail Codon optimized cDNA(gene) Clone

Grail Cat #

RPA0461

Tag

Grail contains A 6x histidine tag

Grail Formulation

Liquid

Grail Storage

Storage at -20℃ to −80℃ Upon receipt. Avoid repeated freeze-thaw cycles.

Grail Applications

Applications: 1. Codon-optimized cDNA(gene) sequence designed for protein production in both E.coli and Mammalian cell. The cDNA(gene) has been cloned into E.coli expression vector with 6x Histidine tag.2. May be used as RNAi assistance to restore the loss-of-function.

Recombinant Grail Protein

Grail Cat #

PA0461

Grail Source

recombinant

Grail Applications

Western Blot, SDS-Page or may be used for other applications determined by user

Grail Purity

>90%, as determined by SDS-PAGE under reducing conditions

Grail Activity

N/A

Tag

Grail Contains A 6x histidine tag

Grail Formulation

Liquid

Grail Storage

;. Storage at -20℃ to −80℃ Upon receipt. Avoid repeated freeze-thaw cycles.

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Grail family

-

Grail Subcellular locations

Endomembrane system; Single-pass membrane protein. Cytoplasm > perinuclear region.

Grail FUNCTION According to UniProtKB Swiss Prot

FUNCTION: E3 ubiquitin-protein ligase that catalyzes polyubiquitin chains. Functions as an inhibitor of cytokine gene transcription. Inhibits IL2 and IL4 transcription and this activity is likely to be mediated by E3 ligase activity. Plays an important role in the induction of the anergic phenotype. Functions in the patterning of the dorsal ectoderm; sensitizes ectoderm to respond to neural-inducing signals (By similarity).

Grail Sequence

DDBJ

EMBL

GenBank

Grail 3D structure

ModBase

Grail interaction

STRING

Grail Proteomic

PRIDE

Grail Genome annotation

Ensembl

GeneID

KEGG

UCSC

Grail Organism specific

CTD

MGI

Grail Phylogenomics

eggNOG

GeneTree

HOGENOM

HOVERGEN

InParanoid

OrthoDB

PhylomeDB

OMA

Grail Gene expression

Bgee

ArrayExpress

Genevestigator

Grail Family and domain

InterPro

PROSITE

ProtoNet

FOR in vitro RESEARCH USE ONLY