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| Mouse V(D)J recombination-activating protein 2 (aa 1-527) |
| Rag-2 Swiss Prot# | | P21784 |
| Rag-2 Protein names | | V(D)J recombination-activating protein 2 (RAG-2) |
| Rag-2 Gene names | | Rag2 (Rag-2) |
| Organism | | Mouse |
| Rag-2 Entry name | | RAG2_MOUSE |
| Rag-2 Codon optimized cDNA(gene) Clone |
| Rag-2 Cat # | | RPW0275 |
| Tag | | Rag-2 contains A 6x histidine tag |
| Rag-2 Formulation | | Liquid |
| Rag-2 Storage | | Storage at -20℃ to −80℃ Upon receipt. Avoid repeated freeze-thaw cycles. |
| Rag-2 Applications | | Applications: 1. Codon-optimized cDNA(gene) sequence designed for protein production in both E.coli and Mammalian cell. The cDNA(gene) has been cloned into E.coli expression vector with 6x Histidine tag.2. May be used as RNAi assistance to restore the loss-of-function. |
| Recombinant Rag-2 Protein |
| Rag-2 Cat # | | PW0275 |
| Rag-2 Source | | recombinant |
| Rag-2 Applications | | Western Blot, SDS-Page or may be used for other applications determined by user |
| Rag-2 Purity | | >90%, as determined by SDS-PAGE under reducing conditions |
| Rag-2 Activity | | N/A |
| Tag | | Rag-2 Contains A 6x histidine tag |
| Rag-2 Formulation | | Liquid |
| Rag-2 Storage | | ;. Storage at -20℃ to −80℃ Upon receipt. Avoid repeated freeze-thaw cycles. |
| Place Order for Recombinant Rag-2 Products | | For pricing and availability Click Here >>> |
| Rag-2 family | | RAG2 family |
| Rag-2 Subcellular locations | | Nucleus. |
| Rag-2 FUNCTION According to UniProtKB Swiss Prot | | FUNCTION: Core component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. DNA cleavage by the RAG complex occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, 5'-phosphorylated ends. The chromatin structure plays an essential role in the V(D)J recombination reactions and the presence of histone H3 trimethylated at 'Lys-4' (H3K4me3) stimulates both the nicking and haipinning steps. The RAG complex also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B lymphocytes. The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. In the RAG complex, RAG2 is not the catalytic component but is required for all known catalytic activities mediated by RAG1. It probably acts as a sensor of chromatin state that recruits the RAG complex to H3K4me3. |
| Rag-2 Sequence | | DDBJ | EMBL | GenBank |
| Rag-2 3D structure | | ModBase |
| Rag-2 interaction | | STRING |
| Rag-2 Proteomic | | PRIDE |
| Rag-2 Genome annotation | | Ensembl | GeneID | KEGG | UCSC |
| Rag-2 Organism specific | | CTD | MGI |
| Rag-2 Phylogenomics | | eggNOG | GeneTree | HOGENOM | HOVERGEN |
| | InParanoid | OrthoDB | PhylomeDB | OMA |
| Rag-2 Gene expression | | Bgee | ArrayExpress | Genevestigator |
| Rag-2 Family and domain | | InterPro | PROSITE | ProtoNet |
| FOR in vitro RESEARCH USE ONLY |
