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Mouse Phosphatidylcholine-sterol acyltransferase (aa 25-438)

Lcat Swiss Prot#

P16301

Lcat Protein names

Phosphatidylcholine-sterol acyltransferase (EC 2.3.1.43) (Lecithin-cholesterol acyltransferase) (Phospholipid-cholesterol acyltransferase)

Lcat Gene names

Lcat

Organism

Mouse

Lcat Entry name

LCAT_MOUSE

Lcat Codon optimized cDNA(gene) Clone

Lcat Cat #

RPW0231

Tag

Lcat contains A 6x histidine tag

Lcat Formulation

Liquid

Lcat Storage

Storage at -20℃ to −80℃ Upon receipt. Avoid repeated freeze-thaw cycles.

Lcat Applications

Applications: 1. Codon-optimized cDNA(gene) sequence designed for protein production in both E.coli and Mammalian cell. The cDNA(gene) has been cloned into E.coli expression vector with 6x Histidine tag.2. May be used as RNAi assistance to restore the loss-of-function.

Recombinant Lcat Protein

Lcat Cat #

PW0231

Lcat Source

recombinant

Lcat Applications

Western Blot, SDS-Page or may be used for other applications determined by user

Lcat Purity

>90%, as determined by SDS-PAGE under reducing conditions

Lcat Activity

N/A

Tag

Lcat Contains A 6x histidine tag

Lcat Formulation

Liquid

Lcat Storage

;. Storage at -20℃ to −80℃ Upon receipt. Avoid repeated freeze-thaw cycles.

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Lcat family

AB hydrolase superfamily, Lipase family

Lcat Subcellular locations

Secreted.

Lcat FUNCTION According to UniProtKB Swiss Prot

FUNCTION: Central enzyme in the extracellular metabolism of plasma lipoproteins. Synthesized mainly in the liver and secreted into plasma where it converts cholesterol and phosphatidylcholines (lecithins) to cholesteryl esters and lysophosphatidylcholines on the surface of high and low density lipoproteins (HDLs and LDLs). The cholesterol ester is then transported back to the liver. Also produced in the brain by primary astrocytes, and esterifies free cholesterol on nascent APOE-containing lipoproteins secreted from glia and influences cerebral spinal fluid (CSF) APOE- and APOA1 levels. Together with APOE and the cholesterol transporter ABCA1, plays a key role in the maturation of glial-derived, nascent lipoproteins. Required for remodeling high-density lipoprotein particles into their spherical forms (By similarity). Has a preference for plasma 16:0-18:2 or 18:O-18:2 phosphatidylcholines.

Lcat Sequence

DDBJ

EMBL

GenBank

Lcat 3D structure

ModBase

Lcat interaction

STRING

Lcat Proteomic

PRIDE

Lcat Genome annotation

Ensembl

GeneID

KEGG

UCSC

Lcat Organism specific

CTD

MGI

Lcat Phylogenomics

eggNOG

GeneTree

HOGENOM

HOVERGEN

InParanoid

OrthoDB

PhylomeDB

OMA

Lcat Gene expression

Bgee

ArrayExpress

Genevestigator

Lcat Family and domain

InterPro

PROSITE

ProtoNet

FOR in vitro RESEARCH USE ONLY