All rights
reserved
|
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
|
||||||||||||||||||||||||||||||||||
|
|||||||||||||
|
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Human Proto-oncogene tyrosine-protein kinase LCK (aa 245–498) |
| LCK Swiss Prot# | | P06239 |
| LCK Protein names | | Proto-oncogene tyrosine-protein kinase LCK (EC 2.7.10.2) (Lymphocyte cell-specific protein-tyrosine kinase) (p56-LCK) (LSK) (T cell-specific protein-tyrosine kinase) |
| LCK Gene names | | LCK |
| Organism | | Human |
| LCK Entry name | | LCK_HUMAN |
| LCK Codon optimized cDNA(gene) Clone |
| LCK Cat # | | RPE0796 |
| Tag | | LCK contains A 6x histidine tag |
| LCK Formulation | | Liquid |
| LCK Storage | | Storage at -20℃ to −80℃ Upon receipt. Avoid repeated freeze-thaw cycles. |
| LCK Applications | | Applications: 1. Codon-optimized cDNA(gene) sequence designed for protein production in both E.coli and Mammalian cell. The cDNA(gene) has been cloned into E.coli expression vector with 6x Histidine tag.2. May be used as RNAi assistance to restore the loss-of-function. |
| Recombinant LCK Protein |
| LCK Cat # | | PE0796 |
| LCK Source | | recombinant |
| LCK Applications | | Western Blot, SDS-Page or may be used for other applications determined by user |
| LCK Purity | | >90%, as determined by SDS-PAGE under reducing conditions |
| LCK Activity | | N/A |
| Tag | | LCK Contains A 6x histidine tag |
| LCK Formulation | | Liquid |
| LCK Storage | | ;. Storage at -20℃ to −80℃ Upon receipt. Avoid repeated freeze-thaw cycles. |
| Place Order for Recombinant LCK Products | | For pricing and availability Click Here >>> |
| LCK family | | Protein kinase superfamily, Tyr protein kinase family, SRC subfamily |
| LCK Subcellular locations | | Cytoplasm. Cell membrane; Lipid-anchor; Cytoplasmic side. |
| LCK FUNCTION According to UniProtKB Swiss Prot | | FUNCTION: Tyrosine kinase that plays an essential role for the selection and maturation of developing T-cell in the thymus and in mature T-cell function. Is constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors and plays a key role in T-cell antigen receptor(TCR)-linked signal transduction pathways. Association of the TCR with a peptide antigen-bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively, and thereby recruits the associated LCK to the vicinity of the TCR/CD3 complex. LCK then phosphorylates tyrosines residues within the immunoreceptor tyrosines-based activation motifs (ITAMs) in the cytoplasmic tails of the TCRgamma chains and CD3 subunits, initiating the TCR/CD3 signaling pathway. In addition, contributes to signaling by other receptor molecules. Associates directly with the cytoplasmic tail of CD2, and upon engagement of the CD2 molecule, LCK undergoes hyperphosphorylation and activation. Also plays a role in the IL2 receptor-linked signaling pathway that controls T-cell proliferative response. Binding of IL2 to its receptor results in increased activity of LCK. Is expressed at all stages of thymocyte development and is required for the regulation of maturation events that are governed by both pre-TCR and mature alpha beta TCR. Phosphorylates RUNX3. |
| LCK Sequence | | DDBJ | EMBL | GenBank |
| LCK 3D structure | | ModBase |
| LCK interaction | | STRING |
| LCK Proteomic | | PRIDE |
| LCK Genome annotation | | Ensembl | GeneID | KEGG | UCSC |
| LCK Organism specific | | CTD | MGI |
| LCK Phylogenomics | | eggNOG | GeneTree | HOGENOM | HOVERGEN |
| | InParanoid | OrthoDB | PhylomeDB | OMA |
| LCK Gene expression | | Bgee | ArrayExpress | Genevestigator |
| LCK Family and domain | | InterPro | PROSITE | ProtoNet |
| FOR in vitro RESEARCH USE ONLY |
