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Human Serine threonine-protein kinase mTOR (aa 2182–2516)

FRAP Swiss Prot#

P42345

FRAP Protein names

Serine/threonine-protein kinase mTOR (EC 2.7.11.1) (Mammalian target of rapamycin) (mTOR) (FKBP12-rapamycin complex-associated protein) (FK506-binding protein 12-rapamycin complex-associated protein 1) (Rapamycin target protein) (RAPT1)

FRAP Gene names

FRAP1 (FRAP) (FRAP2)

Organism

Human

FRAP Entry name

MTOR_HUMAN

FRAP Codon optimized cDNA(gene) Clone

FRAP Cat #

RPE0606

Tag

FRAP contains A 6x histidine tag

FRAP Formulation

Liquid

FRAP Storage

Storage at -20℃ to −80℃ Upon receipt. Avoid repeated freeze-thaw cycles.

FRAP Applications

Applications: 1. Codon-optimized cDNA(gene) sequence designed for protein production in both E.coli and Mammalian cell. The cDNA(gene) has been cloned into E.coli expression vector with 6x Histidine tag.2. May be used as RNAi assistance to restore the loss-of-function.

Recombinant FRAP Protein

FRAP Cat #

PE0606

FRAP Source

recombinant

FRAP Applications

Western Blot, SDS-Page or may be used for other applications determined by user

FRAP Purity

>90%, as determined by SDS-PAGE under reducing conditions

FRAP Activity

N/A

Tag

FRAP Contains A 6x histidine tag

FRAP Formulation

Liquid

FRAP Storage

;. Storage at -20℃ to −80℃ Upon receipt. Avoid repeated freeze-thaw cycles.

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FRAP family

PI3/PI4-kinase family

FRAP Subcellular locations

Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side. Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side. Lysosome. Cytoplasm. Nucleus > PML body.

FRAP FUNCTION According to UniProtKB Swiss Prot

FUNCTION: Kinase subunit of both mTORC1 and mTORC2, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Growth factor-stimulated mTORC1 activation involves AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino-acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-421', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Phosphorylates MAF1 leading to attenuation of its RNA polymerase III-repressive function. mTORC2 is also activated by growth. factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide

exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'.

FRAP Sequence

DDBJ

EMBL

GenBank

FRAP 3D structure

ModBase

FRAP interaction

STRING

FRAP Proteomic

PRIDE

FRAP Genome annotation

Ensembl

GeneID

KEGG

UCSC

FRAP Organism specific

CTD

MGI

FRAP Phylogenomics

eggNOG

GeneTree

HOGENOM

HOVERGEN

InParanoid

OrthoDB

PhylomeDB

OMA

FRAP Gene expression

Bgee

ArrayExpress

Genevestigator

FRAP Family and domain

InterPro

PROSITE

ProtoNet

FOR in vitro RESEARCH USE ONLY