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Human Methylated-DNA-protein-cysteine methyltransferase (aa 1–207)

MGMT Swiss Prot#

P16455

MGMT Protein names

Methylated-DNA--protein-cysteine methyltransferase (EC 2.1.1.63) (6-O-methylguanine-DNA methyltransferase) (MGMT) (O-6-methylguanine-DNA-alkyltransferase)

MGMT Gene names

MGMT

Organism

Human

MGMT Entry name

MGMT_HUMAN

MGMT Codon optimized cDNA(gene) Clone

MGMT Cat #

RPE0465

Tag

MGMT contains A 6x histidine tag

MGMT Formulation

Liquid

MGMT Storage

Storage at -20℃ to −80℃ Upon receipt. Avoid repeated freeze-thaw cycles.

MGMT Applications

Applications: 1. Codon-optimized cDNA(gene) sequence designed for protein production in both E.coli and Mammalian cell. The cDNA(gene) has been cloned into E.coli expression vector with 6x Histidine tag.2. May be used as RNAi assistance to restore the loss-of-function.

Recombinant MGMT Protein

MGMT Cat #

PE0465

MGMT Source

recombinant

MGMT Applications

Western Blot, SDS-Page or may be used for other applications determined by user

MGMT Purity

>90%, as determined by SDS-PAGE under reducing conditions

MGMT Activity

N/A

Tag

MGMT Contains A 6x histidine tag

MGMT Formulation

Liquid

MGMT Storage

;. Storage at -20℃ to −80℃ Upon receipt. Avoid repeated freeze-thaw cycles.

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MGMT family

MGMT family

MGMT Subcellular locations

Nucleus.

MGMT FUNCTION According to UniProtKB Swiss Prot

FUNCTION: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.

MGMT Sequence

DDBJ

EMBL

GenBank

MGMT 3D structure

ModBase

MGMT interaction

STRING

MGMT Proteomic

PRIDE

MGMT Genome annotation

Ensembl

GeneID

KEGG

UCSC

MGMT Organism specific

CTD

MGI

MGMT Phylogenomics

eggNOG

GeneTree

HOGENOM

HOVERGEN

InParanoid

OrthoDB

PhylomeDB

OMA

MGMT Gene expression

Bgee

ArrayExpress

Genevestigator

MGMT Family and domain

InterPro

PROSITE

ProtoNet

FOR in vitro RESEARCH USE ONLY