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Human Macrophage migration inhibitory factor (aa 2–115)

GLIF Swiss Prot#

P14174

GLIF Protein names

Macrophage migration inhibitory factor (MIF) (EC 5.3.2.1) (Phenylpyruvate tautomerase) (L-dopachrome tautomerase) (EC 5.3.3.12) (L-dopachrome isomerase) (Glycosylation-inhibiting factor) (GIF)

GLIF Gene names

MIF (GLIF) (MMIF)

Organism

Human

GLIF Entry name

MIF_HUMAN

GLIF Codon optimized cDNA(gene) Clone

GLIF Cat #

RPE0411

Tag

GLIF contains A 6x histidine tag

GLIF Formulation

Liquid

GLIF Storage

Storage at -20℃ to −80℃ Upon receipt. Avoid repeated freeze-thaw cycles.

GLIF Applications

Applications: 1. Codon-optimized cDNA(gene) sequence designed for protein production in both E.coli and Mammalian cell. The cDNA(gene) has been cloned into E.coli expression vector with 6x Histidine tag.2. May be used as RNAi assistance to restore the loss-of-function.

Recombinant GLIF Protein

GLIF Cat #

PE0411

GLIF Source

recombinant

GLIF Applications

Western Blot, SDS-Page or may be used for other applications determined by user

GLIF Purity

>90%, as determined by SDS-PAGE under reducing conditions

GLIF Activity

N/A

Tag

GLIF Contains A 6x histidine tag

GLIF Formulation

Liquid

GLIF Storage

;. Storage at -20℃ to −80℃ Upon receipt. Avoid repeated freeze-thaw cycles.

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GLIF family

MIF family

GLIF Subcellular locations

Secreted. Cytoplasm.

GLIF FUNCTION According to UniProtKB Swiss Prot

FUNCTION: Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.

GLIF Sequence

DDBJ

EMBL

GenBank

GLIF 3D structure

ModBase

GLIF interaction

STRING

GLIF Proteomic

PRIDE

GLIF Genome annotation

Ensembl

GeneID

KEGG

UCSC

GLIF Organism specific

CTD

MGI

GLIF Phylogenomics

eggNOG

GeneTree

HOGENOM

HOVERGEN

InParanoid

OrthoDB

PhylomeDB

OMA

GLIF Gene expression

Bgee

ArrayExpress

Genevestigator

GLIF Family and domain

InterPro

PROSITE

ProtoNet

FOR in vitro RESEARCH USE ONLY