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Human Ubiquitin-protein ligase E3A (aa 418–517)

E6AP Swiss Prot#

Q05086

E6AP Protein names

Ubiquitin-protein ligase E3A (EC 6.3.2.-) (E6AP ubiquitin-protein ligase) (Oncogenic protein-associated protein E6-AP) (Human papillomavirus E6-associated protein) (Renal carcinoma antigen NY-REN-54)

E6AP Gene names

UBE3A (E6AP) (EPVE6AP) (HPVE6A)

Organism

Human

E6AP Entry name

UBE3A_HUMAN

E6AP Codon optimized cDNA(gene) Clone

E6AP Cat #

RPE0406

Tag

E6AP contains A 6x histidine tag

E6AP Formulation

Liquid

E6AP Storage

Storage at -20℃ to −80℃ Upon receipt. Avoid repeated freeze-thaw cycles.

E6AP Applications

Applications: 1. Codon-optimized cDNA(gene) sequence designed for protein production in both E.coli and Mammalian cell. The cDNA(gene) has been cloned into E.coli expression vector with 6x Histidine tag.2. May be used as RNAi assistance to restore the loss-of-function.

Recombinant E6AP Protein

E6AP Cat #

PE0406

E6AP Source

recombinant

E6AP Applications

Western Blot, SDS-Page or may be used for other applications determined by user

E6AP Purity

>90%, as determined by SDS-PAGE under reducing conditions

E6AP Activity

N/A

Tag

E6AP Contains A 6x histidine tag

E6AP Formulation

Liquid

E6AP Storage

;. Storage at -20℃ to −80℃ Upon receipt. Avoid repeated freeze-thaw cycles.

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E6AP family

-

E6AP Subcellular locations

Nucleus.

E6AP FUNCTION According to UniProtKB Swiss Prot

FUNCTION: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and transfers it to its substrates. Several substrates have been identified including the RAD23A and RAD23B, MCM7 (which is involved in DNA replication), annexin A1, the PML tumor suppressor, and the cell cycle regulator CDKN1B. Additionally, may function as a cellular quality control ubiquitin ligase by helping the degradation of the cytoplasmic misfolded proteins. Finally, UBE3A also promotes its own degradation in vivo.

E6AP Sequence

DDBJ

EMBL

GenBank

E6AP 3D structure

ModBase

E6AP interaction

STRING

E6AP Proteomic

PRIDE

E6AP Genome annotation

Ensembl

GeneID

KEGG

UCSC

E6AP Organism specific

CTD

MGI

E6AP Phylogenomics

eggNOG

GeneTree

HOGENOM

HOVERGEN

InParanoid

OrthoDB

PhylomeDB

OMA

E6AP Gene expression

Bgee

ArrayExpress

Genevestigator

E6AP Family and domain

InterPro

PROSITE

ProtoNet

FOR in vitro RESEARCH USE ONLY