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| Human AP-2 complex subunit mu-1 (aa 1-435) |
| CLAPM1 Swiss Prot# | | Q96CW1 |
| CLAPM1 Protein names | | AP-2 complex subunit mu-1 (Adaptin-mu2) (AP-2 mu-2 chain) (Plasma membrane adaptor AP-2 50 kDa protein) (HA2 50 kDa subunit) (Clathrin assembly protein complex 2 medium chain) (Clathrin coat assembly protein AP50) (Clathrin coat-associated protein AP50) |
| CLAPM1 Gene names | | AP2M1 (CLAPM1) (KIAA0109) |
| Organism | | Human |
| CLAPM1 Entry name | | AP2M1_HUMAN |
| CLAPM1 Codon optimized cDNA(gene) Clone |
| CLAPM1 Cat # | | RPE0196 |
| Tag | | CLAPM1 contains A 6x histidine tag |
| CLAPM1 Formulation | | Liquid |
| CLAPM1 Storage | | Storage at -20℃ to −80℃ Upon receipt. Avoid repeated freeze-thaw cycles. |
| CLAPM1 Applications | | Applications: 1. Codon-optimized cDNA(gene) sequence designed for protein production in both E.coli and Mammalian cell. The cDNA(gene) has been cloned into E.coli expression vector with 6x Histidine tag.2. May be used as RNAi assistance to restore the loss-of-function. |
| Recombinant CLAPM1 Protein |
| CLAPM1 Cat # | | PE0196 |
| CLAPM1 Source | | recombinant |
| CLAPM1 Applications | | Western Blot, SDS-Page or may be used for other applications determined by user |
| CLAPM1 Purity | | >90%, as determined by SDS-PAGE under reducing conditions |
| CLAPM1 Activity | | N/A |
| Tag | | CLAPM1 Contains A 6x histidine tag |
| CLAPM1 Formulation | | Liquid |
| CLAPM1 Storage | | ;. Storage at -20℃ to −80℃ Upon receipt. Avoid repeated freeze-thaw cycles. |
| Place Order for Recombinant CLAPM1 Products | | For pricing and availability Click Here >>> |
| CLAPM1 family | | Adaptor complexes medium subunit family |
| CLAPM1 Subcellular locations | | Cell membrane. Membrane > coated pit; Peripheral membrane protein; Cytoplasmic side. |
| CLAPM1 FUNCTION According to UniProtKB Swiss Prot | | FUNCTION: Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also | play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 mu subunit binds to transmembrane cargo proteins; it recognizes the Y-X-X-Phi motifs. The surface region interacting with to the Y-X-X-Phi motif is inaccessible in cytosolic AP-2, but becomes accessible through a conformational change following phosphorylation of AP-2 mu subunit at 'Tyr-156' in membrane-associated AP-2. The membrane-specific phosphorylation event appears to involve assembled clathrin which activates the AP-2 mu kinase AAK1 (By similarity). |
| CLAPM1 Sequence | | DDBJ | EMBL | GenBank |
| CLAPM1 3D structure | | ModBase |
| CLAPM1 interaction | | STRING |
| CLAPM1 Proteomic | | PRIDE |
| CLAPM1 Genome annotation | | Ensembl | GeneID | KEGG | UCSC |
| CLAPM1 Organism specific | | CTD | MGI |
| CLAPM1 Phylogenomics | | eggNOG | GeneTree | HOGENOM | HOVERGEN |
| | InParanoid | OrthoDB | PhylomeDB | OMA |
| CLAPM1 Gene expression | | Bgee | ArrayExpress | Genevestigator |
| CLAPM1 Family and domain | | InterPro | PROSITE | ProtoNet |
| FOR in vitro RESEARCH USE ONLY |
